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How is Puberty Onset Developmentally Programmed?

Updated: Nov 17

The hypothalamus, one of the most complex brain regions in the mammalian nervous system, contains an astonishing heterogeneity of neurons that regulate endocrine, autonomic and behavioral functions. It not only regulates food consumption, water intake, body temperature, circadian rhythm and sleep to maintain the survival of individual organisms, but also controls puberty onset and reproductive behavior to sustain the breeding population.


“What triggers puberty” is one of the 125 Big Questions posed in the 125th anniversary edition of Science magazine. We have recently revealed that the developmental programming of puberty onset is dependent on TBX3. We have also uncovered new rules for lineage progression, which operate through neuronal differentiation during the development of the hypothalamus. These results were published in Science Advances on Nov. 16.



In this study, we found that TBX3 defines a progenitor domain in the developing hypothalamus and serves as a fate determinant to sequentially control the establishment and maintenance of neuronal fate.


The neuroendocrine system consists of a heterogeneous collection of neuropeptidergic neurons in the brain, among which hypothalamic KNDy neurons represent an indispensable cell subtype controlling puberty onset. Although it has been proposed that hypothalamic neural progenitors and neuronal precursors along the lineage hierarchy adopt a cascade diversification strategy to generate extreme neuronal diversity, the cellular logic for specifying a subtype of neuroendocrine neurons has been unclear.


Previous genetic studies suggest that genetic mutations in TBX3 cause ulnar-mammary syndrome (UMS), which is characterized by shortened forelimbs, defective mammary gland development and genital abnormalities. It is notable that most UMS patients display delayed onset of puberty.


At the organism level, genetic ablation of Tbx3 significantly delays the puberty onset of animals and disturbs the estrous cycle of female mice. At the cellular level, TBX3 plays an important role in the fate establishment and maintenance of hypothalamic KNDy neurons. In addition, at the molecular level, TBX3 regulates gene transcription via phase separation, thereby inducing neuropeptide expression in the hypothalamic neurons.


Importantly, multiple TBX3 mutants identified in UMS patients fail to form phase-separated condensates and cannot efficiently regulate neuropeptide expression, which provides a pathological mechanism underlying the delayed puberty in UMS patients.



Furthermore, we aimed to answer how neuronal lineage progresses during hypothalamus development under physiological and pathological conditions. We used an unprecedented strategy of cell-type alignment by comparing single-cell datasets from lineage tracing and genetically manipulated mice, and revealed two lineage-independent rules—intralineage retention (ILR) and interlineage interaction (ILI)—that regulate lineage progression under pathological conditions.



Collectively, this study uncovers the cellular and molecular mechanisms underlying how TBX3 mutations interfere with the onset of puberty in UMS patients and reveals the rules of ILR and ILI during cell fate specification.


This work was funded by the National Key R&D Program of China, the National Natural Science Foundation of China, the Strategic Priority Research Program of CAS and the Beijing Municipal Science & Technology Commission.

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